Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: Raman images of CCD-18Co cells for control cells and cells incubated with leucine, threonine, and arginine, with corresponding Raman spectra. The colors of spectra correspond to the colors of classes in the Raman maps and represent specific organelles: nucleus (red), lipid droplets (orange), endoplasmic reticulum (blue), mitochondria (magenta), cytoplasm (green), and membrane (light gray).

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Control, Incubation, Membrane

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: Raman images of CCD-18Co cells for control cells and cells incubated with leucine, threonine, and arginine obtained by the integration of the Raman bands over the characteristic spectral regions—organic matter (2820–3030 cm −1 ), nucleus (2935–3005 cm −1 ), saturated lipids (2820–2880 cm −1 ), and unsaturated lipids (3000–3030 cm −1 )—SAT/UNSAT lipid images, and microscope images.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Control, Incubation, Microscopy

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: The average Raman spectra +/− SD (SD—standard deviation) in fingerprint region and in the high-frequency region for lipid droplets obtained for control CCD-18Co (blue line), CCD-18Co supplemented with leucine (olive line), CCD-18Co supplemented with threonine (magenta line), CCD-18Co supplemented with arginine (orange line), control Caco-2 (red line), Caco-2 supplemented with leucine (turquoise line), Caco-2 supplemented with threonine (green line), and Caco-2 supplemented with arginine (gray line). 20 mW laser power, 0.5 s integration time in the fingerprint region, and 0.3 s integration time in the high-frequency region.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Standard Deviation, Control

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: PLS-DA score plots (model: mean center) for Raman spectra for lipid droplets for control CCD-18Co cell line (navy diamond), CCD-18Co cell line supplemented with leucine (blue circle), CCD-18Co cell line supplemented with threonine (ocean square), CCD-18Co cell line supplemented with arginine (turquoise pentagram) and for Raman spectra for control Caco-2 cell line (purple diamond), Caco-2 cell line supplemented with leucine (red circle), Caco-2 cell line supplemented with threonine (magenta square), Caco-2 cell line supplemented with arginine (light pink pentagram), and the loadings plots of LV1–LV3. One point in the PLS-DA score plot represents the average Raman spectrum of lipid droplets in one cell.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques: Control

Journal: Molecules

Article Title: Lipid Droplets as Cellular Sensors of Lipid Metabolic Reprogramming in Colon Cancer: Insights from Essential Amino Acid Supplementation Using Raman Spectroscopy and Imaging

doi: 10.3390/molecules31050762

Figure Lengend Snippet: Response for lipid droplets of normal CCD-18Co (blue bar) colon cells to leucine (olive bar), threonine (magenta bar), and arginine (orange bar) supplementation and of cancer Caco-2 (red bar) colon cells to leucine (turquoise bar), threonine (green bar), and arginine (gray bar) supplementation. Normalized Raman intensities of the ratios: 2845/3015, 2845/2930, 3015/2888, and 1444/1256. *** means statistically significant results, p -value ≤ 0.001; ** means statistically significant results, p -value ≤ 0.01; * means statistically significant results, p -value ≤ 0.05. The number of cells for each culture condition is n = 10.

Article Snippet: CCD-18Co (ATCC ® CRL-1459TM) and Caco-2 cell lines were acquired from ATCC (Manassas, VA, USA) and cultured according to their standard procedures.

Techniques:

Journal: Bioactive Materials

Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair

doi: 10.1016/j.bioactmat.2026.02.002

Figure Lengend Snippet: Therapy platform enhances osteogenic potential of senescent BMSCs in vitro . (A) Representative ALP staining images at day 7 (scale bars: top-10 mm, and bot-200 μm). (B) Quantitative analysis of ALP activity ( n = 3). (C) Representative ARS staining images at day 21 (scale bars: top-5 mm and bot-200 μm). (D) Quantification of mineralized matrix formation via ARS staining ( n = 3). (E-H) qRT-PCR analysis of osteogenic markers ( Alp , Osx , Runx2 , and Opn ) in BMSCs ( n = 3). (I) Representative immunofluorescence images of RUNX2 staining (scale bars: 50 μm). (J) Quantitative analysis of RUNX2 fluorescence intensity ( n = 3). (K) Representative immunofluorescence images of OPN staining (scale bars: 50 μm). (L) Quantitative analysis of OPN fluorescence intensity ( n = 3). Data are presented as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: Protein expression of RUNX2 (Proteintech, 20700-1-AP; 1:500) and OPN (Abcam, ab63856; 1:500) were detected by immunofluorescence staining on day 14, and fluorescence intensities were quantified using ImageJ software (Media Cybernetics).

Techniques: In Vitro, Staining, Activity Assay, Quantitative RT-PCR, Immunofluorescence, Fluorescence

Journal: Bioactive Materials

Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair

doi: 10.1016/j.bioactmat.2026.02.002

Figure Lengend Snippet: In vivo evaluation of the therapeutic platform for aged bone defect repair. (A) Representative 3D-reconstructed micro-CT images of calvarial bone defects in different groups (scale bars: 2 mm). (B-C) Quantification of bone volume fraction (BV/TV) and bone mineral density (BMD) based on micro-CT analysis ( n = 5). (D-E) Representative histological images of H&E staining (D) and Masson's trichrome staining (E). Low-magnification images (scale bars: 500 μm) show the overall defect region, while high-magnification images (scale bars: 100 μm) highlight the new bone (blue box) and defect-host bone interface (red box). Green arrows indicate defect boundaries; FT: fiber tissue; NB: new formed bone; HB: host bone. (F-G) Immunofluorescence images and quantification of RUNX2 expression in defect areas (scale bars: 50 μm, n = 3). (H-I) Immunofluorescence images and quantification of OCN expression (scale bars: 50 μm, n = 3). (J) Schematic illustration of the programmed ROS bidirectional modulation strategy for infection control and repair of aged bone defects, depicting acousto-optic dynamic ROS generation for antimicrobial effects followed by antioxidant modulation to rejuvenate senescent BMSCs and promote aged bone regeneration. Data are expressed as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001.

Article Snippet: Protein expression of RUNX2 (Proteintech, 20700-1-AP; 1:500) and OPN (Abcam, ab63856; 1:500) were detected by immunofluorescence staining on day 14, and fluorescence intensities were quantified using ImageJ software (Media Cybernetics).

Techniques: In Vivo, Micro-CT, Staining, Immunofluorescence, Expressing, Infection, Control